产品细节介绍
3BHB007211R102通道隔离模块
波形捕捉
369在跟踪缓冲区中每次发生跳闸时存储16个A/D采样周期。跟踪内存触发器在S1中设置
首选项并确定存储的行车前和行车后循环数。跟踪缓冲区带有时间和日期标记并可能与事件记录中的跳闸相关。当发生跳闸时，以这种方式捕获7个波形。这是3个相电流、接地电流和3个电压波形。最后三条捕获的记录由369保留
信息存储在易失性存储器中，如果继电器重新通电，信息将丢失。
要访问捕获的波形，请通过写入跟踪内存缓冲区选择器（地址30F5h），然后通过将其跟踪存储器通道（见下表）写入跟踪存储器来选择感兴趣的波形频道选择器（地址30F6h）。然后从地址3100h到31FFh读取跟踪内存数据。读取的值为实际安培或伏特。套件存放如果打算在24小时。否则，检测板、标准品、检测试剂A、检测试剂B应储存在-20°C下。避免重复冻融循环。将所有其他套件组件存放在4°C。这个基板不得冻结。打开单个试剂后建议在1个月内使用该试剂盒。未使用的带材板井应储存在-20°C的密封袋中干燥剂，以尽量减少接触水分。不要使用套件超过有效期。
其他所需供应品
带450nm波长滤波器的微孔板阅读器
高精度移液管和无菌移液管尖端
Eppendorf管
37°C培养箱
去离子水或蒸馏水
吸水纸测试计划
在使用该试剂盒之前，研究人员应考虑以下事项：
1.完整阅读本手册，以尽量减少错误
2.确认您有合适的非供应设备
3.确认该试剂盒的种类、靶抗原和敏感性适合您的预期应用。
4.确认您的样品已准备妥当根据建议（见样品制备）足够的样本量用于分析。
5.首次使用试剂盒时，应采取适当的验证步骤在使用有价值的样品之前。确认套件足够通过以下方法检测预期样本类型中的目标抗原运行控制样本。
6.如果样本中的目标抗原浓度为未知，应使用控件运行初步实验确定最佳样品稀释度的样品（见实验布局和样品制备）。
7.确保套件正确存放，不得超出其使用范围使用到期日期。
8.当使用同一试剂盒的多批次时，不要替换试剂从一个套件到另一个套件。在更改时仔细查看每个手册可以在批次之间发生。控制分析间变异性包括遗留控制样本。
WAVEFORM CAPTURE
The 369 stores 16 cycles of A/D samples each time a trip occurs in a trace buffer. The Trace Memory Trigger is set up in S1 Preferences and determines how many pre-trip and post-trip cycles are stored. The trace buffer is time and date stamped and may be correlated to a trip in the event record. 7 waveforms are captured this way when a trip occurs. These are the 3 phase currents, ground current and 3 voltage waveforms. The last three captured records are retained by the 369. This information is stored in volatile memory and will be lost if power is cycled to the relay. To access the captured waveforms, select the captured record by writing to the Trace Memory Buffer Selector (address 30F5h), then select waveform of interest by writing its trace memory channel (see following table) to the Trace Memory Channel Selector (address 30F6h). Then read the trace memory data from address 3100h to 31FFh. The values read are in actual amperes or volts.
KIT STORAGE
Upon receipt the kit should be stored at 4°C if intended for use within 24 hours. Otherwise the Assay Plate, Standard, Detection Reagent A, and Detection Reagent B should be stored at -20°C. Avoid repeated freeze-thaw cycles. Store all other kit components at 4°C. The Substrate should never be frozen. Once individual reagents are opened it is recommended that the kit be used within 1 month. Unused Strip Plate wells should be stored at -20°C in a sealed bag containing desiccant in order to minimize exposure to moisture. Do not use the kit beyond its expiration date. OTHER REQUIRED SUPPLIES Microplate reader with 450nm wavelength filter High-precision pipette and sterile pipette tips Eppendorf tubes 37°C incubator Deionized or distilled water Absorbent paper
ASSAY PLANNING
Before using this kit, researchers should consider the following: 1. Read this manual in its entirety in order to minimize the chance of error. 2. Confirm that you have the appropriate non-supplied equipment available. 3. Confirm that the species, target antigen, and sensitivity of this kit are appropriate for your intended application. 4. Confirm that your samples have been prepared appropriately based upon recommendations (see Sample Preparation) and that you have sufficient sample volume for use in the assay. 5. When first using a kit, appropriate validation steps should be taken before using valuable samples. Confirm that the kit adequately detects the target antigen in your intended sample type(s) by running control samples. 6. If the concentration of target antigen within your samples is unknown, a preliminary experiment should be run using a control sample to determine the optimal sample dilution (see Experimental Layout and Sample Preparation). 7. Ensure that the kit is properly stored and do not use it beyond its expiration date. 8. When using multiple lots of the same kit do not substitute reagents from one kit to another. Review each manual carefully as changes can occur between lots. To control for inter-assay variability include a carry-over control sample.
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